Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), Super Bright 702, eBioscience
사람 CD42b 단백질을 인식하는 HIP1 단클론 항체로, Super Bright 702 형광체가 결합된 유세포 분석용 시약입니다. 405 nm 레이저로 여기되어 702 nm에서 방출되며, 혈소판 표지 및 응고 관련 연구에 적합합니다. 빛에 민감하므로 암조건에서 보관하세요.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 702, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIP1 |
| Conjugate | Super Bright™ 702 |
| Excitation/Emission Max | 413/702 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762573 |
Available Formats
- Unconjugated
- APC
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Yellow 610
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
Description:
The HIP1 monoclonal antibody reacts with human CD42b (platelet gpIb), a 135 kDa transmembrane protein.
Applications Reported:
HIP1 antibody is reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of human platelets. Recommended usage is 5 µL (0.5 µg) per test (100 µL final volume). Suitable for 10⁵–10⁸ cells/test.
Fluorochrome Characteristics:
Super Bright 702 is a tandem dye excited by the violet laser (405 nm) and emits at 702 nm. Recommended filter: 710/50 bandpass. Ensure instrument compatibility.
Staining Recommendations:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark. Clone-specific performance should be verified empirically.
Excitation/Emission: 405 nm / 702 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD42b is a platelet activation marker involved in coagulation and platelet aggregation. It forms part of the GPIb-IX-V receptor complex, which mediates platelet adhesion to thrombogenic surfaces via von Willebrand factor (VWF). CD42b also interacts with thrombin, coagulation factors XI and XII, TSP-1, integrin Mac-1, and P-selectin. It is expressed on platelets and bone marrow megakaryocytes. Mutations in CD42b are associated with Bernard-Soulier syndrome and platelet-type von Willebrand disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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