Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), Super Bright 702, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 6 publications

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 805
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The eBioA1 monoclonal antibody reacts with human CD39 (ENTPD1 or NTPDase). CD39 is an integral membrane protein with two transmembrane domains and forms a homotetramer. It is the most prominent ectoenzyme of the immune system, converting extracellular ATP to ADP or AMP. CD39 works with CD73 to hydrolyze ATP and is expressed on activated lymphocytes, subsets of T cells, B cells, dendritic cells, and weakly on monocytes and granulocytes.

CD39 and CD73 are found on regulatory T cells (Treg), facilitating their entry into inflamed areas with high ATP levels. Expression of CD39 on Foxp3+CD4+ cells ranges from 25–45%.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted; tested on normal human peripheral blood cells at 5 µL (0.5 µg)/test
  • A test = amount to stain cell sample in 100 µL final volume (10⁵–10⁸ cells/test)

Super Bright 702 is a tandem dye excited by the violet laser (405 nm) and emits at 702 nm. Recommended filter: 710/50 bandpass.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

• Light Sensitivity: Protect from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in the dark.
• Excitation: 405 nm
• Emission: 702 nm
• Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD39 is a 70–100 kDa cell surface antigen expressed on peripheral blood B cells, monocytes, T cell clones, and weakly on granulocytes. It has intrinsic ecto-ATPase activity. Expression increases on activated T and B cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지