Thermo Fisher Scientific Vinculin Monoclonal Antibody (VCL, 3617)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• For Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane.
In adhesion plaque assembly, the β subunit of integrin binds to talin; talin binds to vinculin, which interacts with α-actinin and possibly with itself.
Since α-actinin binds and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.
Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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