Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (sl6arg), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 514
• Alexa Fluor 700
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Request custom conjugation

Product Specific Information

The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that helps eliminate nitrogen by converting L-arginine to urea and L-ornithine, which further converts to polyamines. These polyamines are important for cell proliferation and detoxification from protein degradation byproducts. Expression is found in the liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells.

Expression in macrophages has not been determined in humans, while in mice it is well established in M2 macrophages. Arginase-1 may be expressed in myeloid populations in breast cancer tumors and is typically found in the majority of hepatocellular carcinomas.

Applications Reported: Suitable for immunohistochemical staining (FFPE), microscopy, and immunocytochemistry.
Applications Tested: Tested in IHC (P) using low pH antigen retrieval; optimal at ≤5 µg/mL.

Fluorochrome Information:
eFluor™ 660 is a replacement for Alexa Fluor™ 647. It emits at 659 nm and is excited with the red laser (633 nm). Ensure instrument compatibility.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, the final step in the urea cycle. The resulting polyamines are vital for cell proliferation and detoxification. By degrading arginine, Arginase-1 deprives nitric oxide synthase of its substrate, down-regulating nitric oxide production.

In both human and mouse, Arginase-1 is expressed in the liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells. In humans, expression occurs in blood neutrophils but not CCR3+ granulocytes. In mice, Arginase-1 is a hallmark of alternatively activated (M2a) macrophages. It may also be expressed in tumor-infiltrating myeloid cells and is typically found in hepatocellular carcinomas.

Defects in Arginase-1 cause argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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