Thermo Fisher Scientific AHR Monoclonal Antibody (FF3399), eFluor 660, eBioscience

Applications

Western Blot (WB)

• Tested: Not specified

Flow Cytometry (Flow)

• Recommended dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The FF3399 monoclonal antibody recognizes human aryl hydrocarbon receptor (AHR).
AHR is a ligand-activated transcription factor that mediates the toxic effects of environmental contaminants such as PCBs and TCDD. It also binds naturally occurring compounds in foods and metabolites. AHR resides in the cytoplasm complexed with HSP90, p23, and XAP2/AIP/ARA9. Upon ligand binding, it translocates to the nucleus, dimerizes with ARNT, and binds to the GCGTG consensus sequence in promoter regions of genes like CYP1A1.
AHR is expressed in many cell types, with highest expression in liver, and is involved in Treg and Th17 cell regulation.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis.

Applications Tested:

• Pre-titrated and tested for intracellular staining of Th17-polarized normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) following BestProtocols® Protocol A.
• Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume).
• Compatible with 633 nm red laser detection.
• Filtration: 0.2 µm post-manufacturing filtered.

Fluorochrome Information:
eFluor® 660 is a replacement for Alexa Fluor® 647.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser

Target Information

AHR (Ah Receptor) belongs to a protein family including ARNT (HIF-1 Beta) and Drosophila PER and SIM proteins.
It contains an N-terminal PAS domain (~200 aa) and binds DNA enhancer sequences to initiate transcription of cytochrome P-450 (CYP1A1).
AHR regulates xenobiotic-metabolizing enzymes and binds aromatic hydrocarbons such as polycyclic and halogenated compounds (e.g., TCDD).
In non-ligand activated cells, AHR complexes with HSP90 in the cytoplasm. Upon ligand binding, it transforms into a nuclear DNA-binding form, forming a heterodimer with ARNT.
Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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