Thermo Fisher Scientific SOX2 Monoclonal Antibody (Btjce), eFluor 660, eBioscience
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
50-9811-82 | - | Thermo Fisher Scientific 50-9811-82 SOX2 Monoclonal Antibody (Btjce), eFluor 660, eBioscience 100 ug pk | 재고문의 | pk | 571,000원 | - | 628,100원 | |
50-9811-80 | - | Thermo Fisher Scientific 50-9811-80 SOX2 Monoclonal Antibody (Btjce), eFluor 660, eBioscience 25 ug pk | 재고문의 | pk | 268,000원 | - | 294,800원 |
다른 상품 둘러보기
Applications
Tested Dilution
Publications
Western Blot (WB)
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View 2 publications 2 publications
Immunohistochemistry (IHC)
-
View 10 publications 10 publications
Immunohistochemistry (Paraffin) (IHC (P))
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View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))
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View 2 publications 2 publications
Immunocytochemistry (ICC/IF)
Assay-Dependent
View 13 publications 13 publications
Flow Cytometry (Flow)
0.125 µg/test
View 6 publications 6 publications
Product Specifications
Species Reactivity
Human, Mouse
Published species
Human, Mouse
Host/Isotype
Rat / IgG2a, kappa
Recommended Isotype Control
Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
Btjce
Conjugate
eFluor™ 660 eFluor™ 660 eFluor™ 660
View additional formats
Excitation/Emission Max
651/668 nm View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11219475
Product Specific Information
Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).
Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.
Applications Reported:This btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.
Applications Tested: This btjce antibody has been tested by immunocytochemistry of fixed and permeabilized F9 cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
This btjce has also been tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer and protocol. Please refer to intracellular Staining Protocol on BestProtocols® web page - Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Bacterial expression of region after aa135 (homology between species but not to other family members)
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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