Thermo Fisher Scientific SOX2 Monoclonal Antibody (Btjce), eFluor 660, eBioscience
SOX2 단백질을 인식하는 Rat 모노클로날 항체로, eFluor 660 형광체로 결합되어 있습니다. 인간 및 마우스 시료에 반응하며, Flow cytometry와 ICC/IF에 최적화되어 있습니다. 줄기세포 및 신경발생 연구용으로 적합합니다.
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Thermo Fisher Scientific SOX2 Monoclonal Antibody (Btjce), eFluor 660, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 10 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | View 13 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Btjce |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219475 |
Product Specific Information
The Btjce monoclonal antibody reacts with transcription factor Sox2, a member of the SOX (sex determining region Y-related HMG Box) family. Sox2 is essential for regulating genes controlling mammalian embryogenesis and maintaining pluripotency in embryonic stem cells. It is expressed in early neurogenesis and neural stem cells, suppressing differentiation.
Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis and immunocytochemistry.
Applications Tested: Tested by ICC of fixed and permeabilized F9 cells (≤10 µg/mL). Also tested by intracellular staining and flow cytometry using the Foxp3/Transcription Factor Buffer and protocol (≤0.125 µg/test).
eFluor® 660 is a replacement for Alexa Fluor® 647, emitting at 659 nm and excited by a red laser (633 nm).
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Expression: Bacterial expression of region after aa135.
Target Information
SOX2 is an intronless gene encoding a transcription factor involved in embryonic development and cell fate determination. It is critical for stem-cell maintenance in the central nervous system and regulates gene expression in the stomach. Mutations in SOX2 are associated with bilateral anophthalmia and optic nerve hypoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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