Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Blue 660-120S

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test
Applications Reported: Flow cytometric analysis of normal human peripheral blood cells
Applications Tested: Pre-titrated and validated for use at 5 µL/test (final volume 100 µL)

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 488, 532, 561, 647, 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor, FITC, PE, PE-Cyanine series, PerCP series, Super Bright series, NovaFluor Blue/Red/UV variants, and custom conjugation available.

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha).
CD127 forms a complex with CD132 (common gamma chain) to create the IL-7 receptor (IL-7R), a type I glycoprotein (~75–80 kDa). It is expressed on immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation.

• Function: Mediates IL-7 signaling through Jak/STAT pathways, essential for lymphocyte development and T-cell homeostasis.
• T-reg marker: CD127 expression is down-regulated in CD4+CD25+ regulatory T cells, providing a refined marker for T-reg identification (Foxp3+).
• Blocking: Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7 (Product #14-1079-80).

Each product includes:

1. 1 vial of NovaFluor conjugate
2. 1 vial of CellBlox Plus Blocking Buffer

NovaFluor dyes compatibility:
Not compatible with DNA-intercalating viability dyes (PI, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

Recommended usage of CellBlox Plus Blocking Buffer:
Add 5 µL per sample or per 100 µL of cell suspension (10³–10⁸ cells).
Use in all experiments involving NovaFluor conjugates for optimal staining.

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures with minimized spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD127 (IL-7 Receptor α)
A glycoprotein regulating lymphopoiesis. The active IL-7 receptor is an alpha/gamma chain heterodimer (IL7R).

• Alpha chain: Determines specific signaling events.
• Gamma chain: Activates signal transduction.
  Promotes proliferation of precursor B cells, thymocytes, T-cell progenitors, and mature CD4+/CD8+ T cells.
  Associated diseases include severe combined immunodeficiency (T−/B−/NK+ types).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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