Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Super Bright 780, eBioscience

Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Super Bright 780, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 700
• Brilliant UV 395, 563, 615, 737
• Brilliant Violet 480, 605, 711
• eFluor 660
• NovaFluor Blue 610-70S, 660-120S, 800
• NovaFluor Red 685, 710
• NovaFluor Yellow 690, 810
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645
• Request custom conjugation

Product Specific Information

This 1RNM44N monoclonal antibody recognizes mouse CD170 (Siglec F), a cell surface lectin of the Ig superfamily binding sialic acid-containing glycoconjugates. The cytoplasmic domain includes an ITIM motif initiating intracellular signaling upon ligand binding. Siglec F is mainly expressed on eosinophils and alveolar macrophages, with lower levels on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8 but not expressed on mast cells.

This antibody recognizes a formaldehyde-fixed epitope and is validated for flow cytometric analysis. Tested on mouse thioglycolate-elicited peritoneal exudate cells at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.

Super Bright 780 is a tandem dye excited by the violet laser (405 nm) and emits at 780 nm. Use a 780/60 bandpass filter and ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Compensation values for Super Bright 780 may vary between beads and cells; compensation with cells is recommended. Protect dye and samples from light due to photo-induced oxidation sensitivity.

Samples can be fixed and stored in IC Fixation Buffer (Product # 00-8222) or Fix/Lyse Solution (Product # 00-5333) up to 3 days at 4°C in the dark with minimal brightness loss.

Excitation: 405 nm
Emission: 780 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

SIGLEC5 is a putative adhesion molecule mediating sialic acid-dependent binding. It binds both α2,3- and α2,6-linked sialic acids. Expression is highest in peripheral blood leukocytes, spleen, bone marrow, and lower in lymph node, lung, appendix, placenta, pancreas, and thymus. Found in monocytes and neutrophils, absent in early leukemic myelomonocytic cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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