Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (F38-2E2), Super Bright 780, eBioscience
인간 CD366(TIM3) 단백질을 인식하는 F38-2E2 클론 단일클론 항체로, Super Bright 780 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, 고감도 분석과 낮은 비특이적 결합을 제공합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (F38-2E2), Super Bright 780, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 5 publications |
| ELISA | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 780 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | F38-2E2 |
| Conjugate | Super Bright™ 780 |
| Excitation / Emission Max | 413 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724091 |
Available Formats
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 805
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 786
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
This F38-2E2 monoclonal antibody reacts with human CD366 (TIM3 or HAVCR2). CD366 is expressed on activated CD4+ T cell subsets (Th1, Th17, Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells. It exists as membrane-bound and soluble forms due to alternative splicing. Galectin-9 is its ligand, and CD366 negatively regulates CD4+ T cells, influencing Th1 and Th17 cytokine secretion. It is implicated in tolerance, autoimmune disease (e.g., multiple sclerosis), and HIV infection.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometric analysis of stimulated human peripheral blood cells at 5 µL (0.06 µg)/test.
A test corresponds to staining a cell sample (10⁵–10⁸ cells) in 100 µL volume.
Super Bright 780 is a tandem dye excited at 405 nm and emits at 780 nm. Recommended filter: 780/60 bandpass. Ensure instrument compatibility.
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Compensation Notes:
Higher compensation values may occur in the violet 450/50 channel when using UltraComp eBeads (Product # 01-2222-42) or AbC Total Antibody Compensation beads (Product # A10497). Compensation with cells is recommended.
Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333). Clone-specific performance should be tested empirically.
Excitation: 405 nm
Emission: 780 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
TIM3 (HAVCR2) is a 281 amino acid, Type-1 Th1-specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. It includes an IgV-like domain, a mucin-like domain, and a conserved tyrosine phosphorylation motif. TIM3 participates in macrophage activation, autoimmune disease induction, and immune tolerance regulation. It is associated with experimental autoimmune encephalomyelitis (EAE) and Th1-dependent autoimmune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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