Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (F38-2E2), Super Bright 780, eBioscience

Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (F38-2E2), Super Bright 780, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 805
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

This F38-2E2 monoclonal antibody reacts with human CD366 (TIM3 or HAVCR2). CD366 is expressed on activated CD4+ T cell subsets (Th1, Th17, Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells. It exists as membrane-bound and soluble forms due to alternative splicing. Galectin-9 is its ligand, and CD366 negatively regulates CD4+ T cells, influencing Th1 and Th17 cytokine secretion. It is implicated in tolerance, autoimmune disease (e.g., multiple sclerosis), and HIV infection.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometric analysis of stimulated human peripheral blood cells at 5 µL (0.06 µg)/test.
A test corresponds to staining a cell sample (10⁵–10⁸ cells) in 100 µL volume.

Super Bright 780 is a tandem dye excited at 405 nm and emits at 780 nm. Recommended filter: 780/60 bandpass. Ensure instrument compatibility.

When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Compensation Notes:
Higher compensation values may occur in the violet 450/50 channel when using UltraComp eBeads (Product # 01-2222-42) or AbC Total Antibody Compensation beads (Product # A10497). Compensation with cells is recommended.

Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333). Clone-specific performance should be tested empirically.

Excitation: 405 nm
Emission: 780 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

TIM3 (HAVCR2) is a 281 amino acid, Type-1 Th1-specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. It includes an IgV-like domain, a mucin-like domain, and a conserved tyrosine phosphorylation motif. TIM3 participates in macrophage activation, autoimmune disease induction, and immune tolerance regulation. It is associated with experimental autoimmune encephalomyelitis (EAE) and Th1-dependent autoimmune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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