Thermo Fisher Scientific CD140b (PDGFRB) Monoclonal Antibody (APB5), Super Bright 780, eBioscience

Thermo Fisher Scientific CD140b (PDGFRB) Monoclonal Antibody (APB5), Super Bright 780, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available:

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Custom conjugation available upon request

Product Specific Information

The APB5 monoclonal antibody reacts with mouse CD140b, the beta chain of the platelet-derived growth factor receptor (PDGFRβ). PDGFRβ is a receptor tyrosine kinase that dimerizes upon ligand binding and phosphorylates substrates. CD140b is expressed in embryonic tissues and mesenchymal-derived adult mouse cells, playing a significant role in fibrous atherosclerotic lesion formation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on NIH/3T3 cells at ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration recommended.

Super Bright 780 is a tandem dye excited by the violet laser (405 nm) and emits at 780 nm. Recommended filter: 780/60 bandpass. Ensure instrument compatibility.

When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness or FRET efficiency.

Excitation: 405 nm
Emission: 780 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

PDGFRB is a receptor tyrosine kinase involved in atherogenesis and cell transformation by the SIS oncogene. It acts as a mitogen for mesenchymal-origin cells. The receptor can form homodimers or heterodimers with PDGFRA depending on ligand binding. The gene resides on chromosome 5 near GM-CSF and M-CSFR genes, implicated in 5-q syndrome. A translocation between chromosomes 5 and 12 fusing PDGFRB and ETV6 leads to chronic myeloproliferative disorder with eosinophilia.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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