Thermo Fisher Scientific IDH1 Recombinant Rabbit Monoclonal Antibody (5S2U7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5S2U7

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human IDH1 (NP_0058872) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IDH1,uniProtId:O75874-1,ncbiNodeId:9606,antigenRange:100-200,antigenLength:414,antigenImageFileName:MA5-42872_IDH1_O75874-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42872_IDH1_O75874-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.08 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2912013

Product Specific Information

Positive test controls include: 293T, HepG2, A-549, MCF7, Mouse testis, Mouse brain, Mouse kidney, Rat liver. The target is usually found in the following locations: Cytoplasm, Peroxisome.

Immunogen sequence: RNILGGTVFR EAIICKNIPR LVSGWVKPII IGRHAYGDQY RATDFVVPGP GKVEITYTPS DGTQKVTYLV HNFEEGGGVA MGMYNQDKSI EDFAHSSFQM A

Target Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.