Thermo Fisher Scientific JunD Recombinant Rabbit Monoclonal Antibody (5L7Y3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin

-

CUT&RUN (C&R)

105 cells /1 µg

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5L7Y3

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human JunD (P17535). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:JunD,uniProtId:P17535-1,ncbiNodeId:9606,antigenRange:100-200,antigenLength:347,antigenImageFileName:MA5-42876_JunD_P17535-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42876_JunD_P17535-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2912017

Product Specific Information

Positive test controls include: HeLa, HT-1080. The target is usually found in the following locations: Nucleus.

Immunogen sequence: SPELERLIIQ SNGLVTTTPT SSQFLYPKVA ASEEQEFAEG FVKALEDLHK QNQLGAGAAA AAAAAAAGGP SGTATGSAPP GELAPAAAAP EAPVYANLSS Y

Target Information

JunD is a transcription factor which is coded by JunD gene. JunD transcriptional activity is modulated by phosphorylation in response to cellular stress via the c-Jun N terminal kinase (JNK)/Stress-Activated Protein Kinase (SAPK) family of protein kinase. Jun proteins are capable of forming dimers with Fos, ATF and CREB family transcription factors to form the AP-1 complex that differentially regulates a variety of target genes involved in cellular growth, proliferation, differentiation and apoptosis. Also, JunD plays an important role in metabolism via modulation of IGF-1 signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.