Thermo Fisher Scientific 3-Nitrotyrosine Monoclonal Antibody (2A12)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2A12

Immunogen

3-Nitrotyrosine-KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS, pH 7.5, with 50% glycerol

Contains

3mM sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_1069883

Target Information

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxidesignaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimers disease, Parkinsons disease and amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.