Thermo Fisher Scientific 3-Nitrotyrosine Monoclonal Antibody (2A12)

Applications

• Western Blot (WB): Assay-dependent

Product Specifications

Target Information

Protein tyrosine nitration is a post-translational modification increasingly recognized as a key component of nitric oxide signaling. While multiple nonenzymatic mechanisms can produce nitrated tyrosine residues, most nitration events are catalyzed by metalloproteins such as myeloperoxidase, eosinophil peroxidase, myoglobin, cytochrome P-450s, superoxide dismutase, and prostacyclin synthase.

Studies show that protein tyrosine nitration is selective and limited to specific proteins. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages results in nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mM CO₂ leads to additional nitration at tyrosine 161.

Nitrotyrosine-containing proteins have been correlated with disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.