Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (TE111.5D11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 16 publications 16 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mL protein lysate

View 8 publications 8 publications

ChIP assay (ChIP)

Assay-dependent

View 21 publications 21 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Hamster, Human, Mouse, Sheep, Pig, Rabbit, Rat

Published species

Bovine, Chicken, Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TE111.5D11

Immunogen

C-terminal fragment (aa 302-595) of human ER expressed in E coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Estrogen Receptor alpha,uniProtId:P03372-1,ncbiNodeId:9606,antigenRange:302-595,antigenLength:595,antigenImageFileName:MA1-12692_Estrogen_Receptor_alpha_P03372-1_House_mouse.svg,antigenImageFileNamePDP:MA1-12692_Estrogen_Receptor_alpha_P03372-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074278

Product Specific Information

A suggested positive control for this product is T47D cells.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.