
Thermo Fisher Scientific PARP1 Monoclonal Antibody (C.384.8)
PARP1 단백질 검출용 Rabbit 모노클로날 항체로, Western blot, ICC/IF, IP, ChIP 등에 적합. PARP-2 및 PARP-3과 교차 반응 없음. 고순도 액상 형태로 제공되며 -20°C 보관. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 5 publications |
| Immunocytochemistry (ICC/IF) | 1:800 | - |
| Immunoprecipitation (IP) | 1:200 | - |
| ChIP assay (ChIP) | 4 µL/10^6 cells | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C.384.8 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Gly623 of human PARP-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 438 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_11000218 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with PARP-2 and PARP-3.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyze the transfer of ADP-ribose to a variety of nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein.
Cleavage occurs between Asp216 and Gly217, a site in PARP conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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