Thermo Fisher Scientific PARP1 Monoclonal Antibody (C.384.8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:800

-

Immunoprecipitation (IP)

1:200

-

ChIP assay (ChIP)

4 µL/10^6 cells

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

C.384.8

Immunogen

Synthetic peptide corresponding to residues surrounding Gly623 of human PARP-1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PARP1,uniProtId:P09874-1,ncbiNodeId:9606,antigenRange:623,antigenLength:1014,antigenImageFileName:MA5-15031_PARP1_P09874-1_Rabbit.svg,antigenImageFileNamePDP:MA5-15031_PARP1_P09874-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

438 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_11000218

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with PARP-2 and PARP-3.

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.