Thermo Fisher Scientific APIP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human APIP (NP_057041.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:APIP,uniProtId:Q96GX9-1,ncbiNodeId:9606,antigenRange:1-242,antigenLength:242,antigenImageFileName:PA5-121067_APIP_Q96GX9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-121067_APIP_Q96GX9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.85 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914639

Product Specific Information

Positive test controls include: 22Rv1, Mouse pancreas, Mouse stomach, Mouse heart, Mouse liver, Rat stomach. The target is usually found in the following locations: Cytoplasm.

Immunogen sequence: MSGCDAREGD CCSRRCGAQD KEHPRYLIPE LCKQFYHLGW VTGTGGGISL KHGDEIYIAP SGVQKERIQP EDMFVCDINE KDISGPSPSK KLKKSQCTPL FMNAYTMRGA GAVIHTHSKA AVMATLLFPG REFKITHQEM IKGIKKCTSG GYYRYDDMLV VPIIENTPEE KDLKDRMAHA MNEYPDSCAV LVRRHGVYVW GETWEKAKTM CECYDYLFDI AVSMKKVGLD PSQLPVGENG IV

Target Information

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf 1 (for apoptosis protease-activating factor 1). Apaf 1 binds to cytochrome c (Apaf 2) and Caspase-9 (Apaf 3), which leads to Caspase-9 activation. Activated Caspase-9 in turn cleaves and activates Caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Recently, Cho et al (4) have identified a new Apaf 1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf 1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.