Thermo Fisher Scientific HSP90 Monoclonal Antibody (H9010), APC
HSP90 단백질을 인식하는 마우스 모노클로날 항체로 APC 형광 표지. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. 인간을 포함한 여러 종에서 반응하며, 단백질 접힘 및 스트레스 반응 연구에 유용.
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Thermo Fisher Scientific HSP90 Monoclonal Antibody (H9010), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:10,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Chicken, Fish, Hamster, Human, Mouse, Rabbit, Rat, Shark |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H9010 |
| Immunogen | Recombinant human HSP90beta |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931556 |
Additional Formats
- FITC (Catalog: MA5-45103)
- PerCP (Catalog: MA5-45104)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45102 was sufficient for detection of HSP90beta in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects 90 kDa. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.
Target Information
HSP90 proteins are highly conserved molecular chaperones involved in signal transduction, protein folding, degradation, and morphologic evolution. They associate with cochaperones to fold newly synthesized proteins or refold denatured proteins after stress. There are two major cytosolic forms: HSP90AA1 (inducible) and HSP90AB1 (constitutive), as well as mitochondrial forms (Chen et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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