Thermo Fisher Scientific HSP90 Monoclonal Antibody (H9010), FITC
FITC로 표지된 HSP90 단클론 항체로 다양한 종에서 반응하며, WB, IHC, ICC 등 여러 응용에 적합합니다. 단백질 접힘 및 스트레스 반응 연구에 유용하며, 고순도 Protein G 정제 및 안정적 형광 신호를 제공합니다.
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Thermo Fisher Scientific HSP90 Monoclonal Antibody (H9010), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:10,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Chicken, Fish, Hamster, Human, Mouse, Rabbit, Rat, Shark |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H9010 |
| Immunogen | Recombinant human HSP90beta |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.09mM sodium bicarbonate, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931557 |
Product Specific Information
1 µg/mL of MA5-45103 was sufficient for detection of HSP90beta in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects 90 kDa. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.
Target Information
HSP90 proteins are highly conserved molecular chaperones involved in signal transduction, protein folding, degradation, and morphologic evolution. They associate with cochaperones to fold newly synthesized proteins or refold denatured proteins after stress.
There are two major cytosolic HSP90 proteins: HSP90AA1 (inducible form) and HSP90AB1 (constitutive form). (Chen et al., 2005)
Additional Formats
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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