Thermo Fisher Scientific AGO2 Recombinant Rabbit Monoclonal Antibody (JF0992)
AGO2 단백질을 검출하기 위한 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. 높은 특이성과 재현성을 제공하며, lot 간 일관성이 우수합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:4,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF0992 |
| Immunogen | Synthetic peptide within Human Argonaute 2 (aa 361–404) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809797 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Clones are screened to select the best candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
Ago proteins are broadly expressed in somatic cells and associate with miRNAs, playing key roles in RNA silencing pathways. Among them, Ago2 uniquely possesses endonucleolytic “Slicer” activity, enabling miRNA-directed cleavage of target mRNA when base-pairing is perfect.
In cases of partial complementarity, Ago2 represses translation rather than cleaving mRNA. It is essential in RNA interference, translation repression, and heterochromatin formation. As a core component of the RISC complex, AGO2 mediates mRNA degradation guided by siRNAs or miRNAs and also functions in Dicer-independent RNA slicing and miRNA maturation regulation.
Overexpression of Ago2 has been linked to cancer progression and patient survival, while gene disruption studies in mice demonstrate its essential role in embryonic, B-lymphoid, and erythroid development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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