Thermo Fisher Scientific S100A10 Recombinant Rabbit Monoclonal Antibody (JF0987)
Recombinant rabbit monoclonal antibody (clone JF0987) targeting S100A10 protein. Validated for WB, IHC-P, and ICC/IF applications. Offers high specificity, lot-to-lot consistency, and animal origin-free formulation. Suitable for research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF0987 |
| Immunogen | Synthetic peptide within Human S100A10 (aa 19–68) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809796 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production. These recombinant antibodies offer enhanced specificity and sensitivity, consistent performance across lots, animal origin-free formulations, and broad immunoreactivity due to the diverse rabbit immune repertoire.
Target Information
The S-100 family of calcium-activated proteins interacts with various target proteins to modulate biological signaling pathways. Many cancer cell lines overexpress the plasminogen receptor S-100A10 on the extracellular surface, forming a heterotetrameric complex with Annexin II. This complex facilitates plasminogen binding and activation but is not required for membrane localization. S-100A10 also acts as a cellular chaperone for hepatitis B virus polymerase, promoting its nuclear translocation and potentially influencing viral replication through calcium-mediated mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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