Thermo Fisher Scientific NKG2A/C/E Monoclonal Antibody (20d5), Biotin, eBioscience
마우스 NKG2A/C/E 인식용 Rat IgG2a 단클론 항체로 Biotin 결합 형태. Flow Cytometry에 최적화되어 있으며 0.5 µg/test 권장. PBS 기반 저장용액, 4°C 보관, 암조건 유지. 연구용으로 NK 세포 및 NKT 세포 분석에 활용 가능.
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Thermo Fisher Scientific NKG2A/C/E Monoclonal Antibody (20d5), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | View 5 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20d5 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466776 |
Additional Formats
Product Specific Information
The 20d5 monoclonal antibody reacts with mouse NKG2A, C, and E. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. These molecules form heterodimeric complexes with CD94 and recognize non-classical MHC class I antigen Qa-1.
When co-staining with NKG2A^B6 clone 16a11, stain for NKG2A-B6 first, then subsequently with NKG2A/C/E to avoid steric hindrance.
Applications Reported: 20d5 is suitable for flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.5 µg per test (100 µL final volume). Optimal antibody titration is recommended.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Natural killer (NK) cells mediate lysis of tumor and virus-infected cells and regulate immune responses. The encoded protein belongs to the NKG2 family of killer cell lectin-like receptors, characterized by type II membrane orientation and a C-type lectin domain. It forms a complex with KLRD1/CD94 and recognizes MHC class I HLA-E molecules. NKG2 family genes are located on chromosome 12, with multiple alternatively spliced variants observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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