Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (LDK18), eBioscience
인간 Cytokeratin 18을 인식하는 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. LDK18 클론 기반으로 높은 순도(>90%)와 낮은 응집도(<10%)를 보유. 4°C 보관, PBS 버퍼 및 0.09% sodium azide 포함. 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LDK18 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865469 |
Additional Formats
- Alexa Fluor 488 (Cat. No. 53-9815-82)
- eFluor 570 (Cat. No. 41-9815-82)
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody LDK18 recognizes human Cytokeratin 18, a member of the intermediate filament family found in epithelial cells. Cytokeratin 18 (type I) typically pairs with Cytokeratin 8 (type II) to form keratin filaments. Because many epithelial-derived tumors retain keratin expression, Cytokeratin 18 serves as a valuable tumor marker for identifying primary and metastatic epithelial tumors. It is also expressed in highly proliferative retinal pigment epithelial cells.
Applications Reported: Western blotting, microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested: Tested by ICC of MeOH-fixed human cells; recommended ≤1 µg/mL. Optimize dilution for specific assays.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) is a type A (acidic) low molecular weight keratin, typically paired with Cytokeratin 8. It is expressed in tissues of the gastrointestinal, respiratory, and urogenital tracts, as well as in endocrine, exocrine, and mesothelial cells. Cytokeratin 18 is prevalent in non-squamous epithelia and most adenocarcinomas and ductal carcinomas, but absent in squamous cell carcinomas. Hepatocellular carcinomas express only Cytokeratins 8 and 18. Mutations in the KRT18 gene are associated with cryptogenic cirrhosis. Two transcript variants encode the same protein.
Cytokeratin subtype expression patterns are increasingly used to distinguish epithelial malignancies. Cytokeratin antibodies assist in tumor differential diagnosis (IHC, cytopathology, flow cytometry).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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