Thermo Fisher Scientific Blood Group Antigen H (O) Type 1 Monoclonal Antibody (17-206), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Alexa Fluor 488 (Cat. No. 53-9810-82)
• Biotin (Cat. No. 13-9810-82)
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody 17-206 recognizes the human blood group antigen H (O) type 1, a subtype primarily found in epithelial and secretory cells. The precursor H antigen is modified by addition of saccharides to carbohydrate chains of proteins and lipids on erythrocytes and epithelial cells. The type, attachment, location, and sequence of saccharides determines the blood group antigen subtype and is mediated by multiple glycosyl transferases. Depending on ABO blood type, the H antigen is converted into either the A antigen, B antigen, both A and B antigens, or left unconverted (O antigen).

Evidence indicates that blood group antigen H is not expressed in tumor cells and that expression level correlates with disease progression, suggesting potential roles in cell proliferation, adhesion, and angiogenesis. The 17-206 antibody does not recognize blood group antigen H (O) type 2.

Applications Reported:

• Immunohistochemical staining of formalin-fixed paraffin-embedded (FFPE) tissue sections
• ELISA

Applications Tested:
Tested by IHC on FFPE human tissue using high or low pH antigen retrieval at ≤5 µg/mL. It is recommended to titrate the antibody for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The H antigen is a precursor to the ABO blood group antigens, present in individuals of all common blood types. The Bombay phenotype (hh) lacks antigen H on red blood cells and therefore also lacks A or B antigens. These individuals produce isoantibodies to antigen H as well as to A and B antigens, making blood transfusion compatibility highly specific to hh donors.

Production of the H antigen, or its deficiency in the Bombay phenotype, is controlled at the H locus on chromosome 19. The H locus encodes a fucosyltransferase responsible for producing the H antigen on RBCs. The H antigen is a carbohydrate sequence primarily linked to proteins, with a minor fraction attached to ceramide moieties.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.