Thermo Fisher Scientific GP6 Monoclonal Antibody (HY101), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Flow Cytometry (Flow)

0.25 µg/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

HY101

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• eFluor 660
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865467

Product Specific Information

Description: The HY101 monoclonal antibody reacts with the glycoprotein VI (GPVI), a 62-kDa type-I transmembrane glycoprotein that is expressed on mature megakaryotes and platelets. GPVI associates with the Fc receptor (FcR) gamma-chain to form a high affinity receptor for collagen. GPVI has also been reported to bind convulxin (Cvx). Phosphorylation of the ITAMs present on the FcR gamma-chain leads to recruitment of Src family kinases and activation of downstream signal transduction. GPVI has been reported to play a critical role in platelet activation and aggregation.

Applications Reported: This HY101 antibody has been reported for use in flow cytometric analysis, and western blotting.

Applications Tested: This HY101 antibody has been tested by flow cytometric analysis or immunoblotting. In flow cytometry this can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Glycoprotein VI (GP6) is a 58-kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. Upon injury to the vessel wall and subsequent damage to the endothelial lining, exposure of the subendothelial matrix to blood flow results in deposition of platelets. Collagen fibers are the most thrombogenic macromolecular components of the extracellular matrix, with collagen types I, III, and VI being the major forms found in blood vessels. Platelet interaction with collagen occurs as a 2-step procedure: (1) the initial adhesion to collagen is followed by (2) an activation step leading to platelet secretion, recruitment of additional platelets, and aggregation. In physiologic conditions, the resulting platelet plug is the initial hemostatic event limiting blood loss. However, exposure of collagen after rupture of atherosclerotic plaques is a major stimulus of thrombus formation associated with myocardial infarction or stroke.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.