Thermo Fisher Scientific Phospho-EGFR (Tyr992) Recombinant Rabbit Monoclonal Antibody (3H24L5)
EGFR의 Tyr992 인산화 부위를 특이적으로 인식하는 재조합 토끼 단클론 항체. 높은 특이성과 재현성을 제공하며, 세포 내 인산화 신호 연구에 적합. Protein A 정제, PBS 완충액에 보관. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3H24L5 |
| Immunogen | Peptide corresponding to Human EGFR (aa 1012–1020) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2632964 |
Product Specific Information
This antibody is predicted to react with Monkey, Cat, and Rabbit.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, and individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to growth factors. Overexpression of EGFR has been linked to various cancers and is the target of multiple therapeutic agents.
EGFR is encoded by the EGFR gene on chromosome 7 and belongs to the HER/ErbB family, which includes ERbB2, ERbB3, and ERbB4. Ligand binding (e.g., EGF, TGF-α) induces receptor dimerization and autophosphorylation of cytoplasmic tyrosine residues, initiating downstream signaling pathways. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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