Thermo Fisher Scientific PARP1 Recombinant Rabbit Monoclonal Antibody (8H2L9)
PARP1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot과 면역세포염색에 적합. 높은 특이성과 민감도, 로트 간 일관성 제공. 인간에 반응하며 원숭이, 돼지, 토끼에도 예측 반응. 단백질 A 정제, 4°C 단기 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8H2L9 |
| Immunogen | Peptide corresponding to Human PARP1 (aa 215–225) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2633085 |
Product Specific Information
This antibody is predicted to react with Monkey, Pig, and Rabbit.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Specific antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal-origin-free formulations
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Poly(ADP-Ribose) Polymerase (PARP) uses NAD⁺ as a substrate to catalyze the transfer of ADP-ribose to nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of apoptosis, mediated by caspase CPP32.
Cleavage occurs between Asp216 and Gly217, a conserved site across species.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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