Thermo Fisher Scientific POLB Recombinant Rabbit Monoclonal Antibody (JM93-12)
Thermo Fisher Scientific의 POLB Recombinant Rabbit Monoclonal Antibody (JM93-12)는 DNA Polymerase β를 표적하는 고특이성 항체로, WB, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. 재조합 기술로 생산되어 로트 간 일관성이 높고 민감도가 우수합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:20–1:50 |
| Immunoprecipitation (IP) | 1:10–1:20 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM93-12 |
| Immunogen | Synthetic peptide within Human DNA Polymerase beta (aa 286–335) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810001 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to the larger rabbit immune repertoire
Target Information
DNA polymerase beta comprises an amino-terminal 8 kDa domain and a carboxy-terminal 31 kDa domain.
- The N-terminal ssDNA binding domain has deoxyribose phosphodiesterase activity.
- The C-terminal domain has nucleotidyltransferase activity.
Mammalian DNA polymerase beta is a DNA repair polymerase constitutively expressed in cultured cells. Treatment of cells with DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol levels.
It fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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