Thermo Fisher Scientific CD1a Recombinant Rabbit Monoclonal Antibody (JM21-33)
Recombinant rabbit monoclonal antibody recognizing human CD1a. Validated for WB and IHC(P). Offers high specificity, lot-to-lot consistency, and excellent sensitivity. Supplied as liquid, 1 mg/mL, Protein A purified. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM21-33 |
| Immunogen | Synthetic peptide within Human CD1a (aa 16–65) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810004 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
CD1a is a non-polymorphic MHC Class I-related cell surface glycoprotein expressed in association with β2-microglobulin. It is found on cortical thymocytes, Langerhans cells, interdigitating cells, certain T-cell leukemias, and in histiocytosis X.
CD1a proteins mediate the presentation of lipid and glycolipid antigens to T cells. The human genome contains five CD1 family genes (CD1a–e) located on chromosome 1, differing in localization and ligand specificity. CD1a localizes to the plasma membrane and early endocytic vesicles. Alternative splicing variants have been observed, though their biological significance is unclear.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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