Thermo Fisher Scientific SMAD4 Recombinant Rabbit Monoclonal Antibody (BLR133J)
SMAD4 단백질을 표적하는 Thermo Fisher Scientific의 재조합 단일클론 토끼 항체입니다. Western blot, IHC, ICC/IF, Flow Cytometry, IP 등 다양한 응용에 적합하며, 높은 특이성과 재현성을 제공합니다. Human 및 Mouse 시료에 반응하며 연구용으로 사용됩니다.
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Thermo Fisher Scientific SMAD4 Recombinant Rabbit Monoclonal Antibody (BLR133J)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 0.25 µL/10⁶ cells |
| Immunoprecipitation (IP) | 6 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR133J |
| Immunogen | Peptide representing a region between residue 1 and 50 of Human SMAD4 (Mothers against decapentaplegic homolog 4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE cell sections.
Target Information
Smad4 is encoded by the Smad4 gene located on human chromosome 18. It is also known as Smad family member 4, Sma- and Mad-related protein 4, or Mothers against decapentaplegic homolog 4.
Smad4 contains N-terminal MH1 and C-terminal MH2 globular domains involved in DNA binding and protein interactions.
Upon ligand binding of the TGF-beta superfamily (including TGF-beta and BMP), receptor-mediated phosphorylation of R-Smads (Smad1, 2, 3, 5, and 8) occurs, leading to heterotrimerization with Smad4 and nuclear translocation.
Smad4 functions as a tumor suppressor by regulating target genes such as Cyclin D1 (downregulation) and collagen (upregulation), which inhibit cell proliferation.
Dephosphorylation controls nuclear export and nucleocytoplasmic dynamics of Smads.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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