Thermo Fisher Scientific SMAD2 Recombinant Rabbit Monoclonal Antibody (BLR130H)
SMAD2 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry, IP 등 다양한 응용에 적합. 인간 및 생쥐 반응성. HEK293F 발현 시스템에서 생산된 고순도 액상 항체.
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Thermo Fisher Scientific SMAD2 Recombinant Rabbit Monoclonal Antibody (BLR130H)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL/10⁶ cells |
| Immunoprecipitation (IP) | 20 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR130H |
| Immunogen | Peptide representing a region between residue 225 and 275 of Human SMAD2 (Mothers against decapentaplegic homolog 2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 500 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes including proliferation, apoptosis, and differentiation. It interacts with TGF-beta receptors via SMAD anchor for receptor activation (SARA) and plays a central role in TGF-beta signaling. Upon phosphorylation, SMAD2 dissociates from SARA and associates with SMAD4, enabling nuclear translocation and transcriptional regulation. It can also be phosphorylated by activin type 1 receptor kinase, mediating activin signaling. Alternative splicing variants encoding SMAD2 have been observed.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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