Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, PeproTech
Goat polyclonal antibody specific for human IL-12 p70. Validated for ELISA and Western blot applications. Affinity purified for high specificity and sensitivity. Suitable for detecting IL-12 in research on immune response and cytokine signaling.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | 2 publications |
| Neutralization (Neu) | - | 1 publication |
| In vitro Assay (IV) | - | 5 publications |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHO cells-derived Recombinant Human IL-12 p70 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929515 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein
- p35 subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS
- p40 subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human IL-12. Anti-Human IL-12-specific antibody was purified by affinity chromatography employing an immobilized Human IL-12 matrix.
Sandwich ELISA:
To detect Human IL-12 by sandwich ELISA (100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. When paired with PeproTech Biotinylated Anti-Human IL-12 (500-P154GBt) as detection antibody, the detection limit is 0.2–0.4 ng/well of Recombinant Human IL-12.
Western Blot:
For Western blot detection of Human IL-12, use 0.1–0.2 µg/mL. Detection limit for Recombinant Human IL-12 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
IL-12 p70 (Interleukin-12)
IL-12 p70 is a cytokine critical in immune response to infections and cancer. Produced by dendritic cells, macrophages, and B cells, it stimulates interferon-gamma production, T cell, and NK cell activation. IL-12 p70 is investigated as a therapeutic target in infectious diseases, autoimmune disorders, and cancer for its role in enhancing immune responses and modulating inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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