Thermo Fisher Scientific HDAC2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.4 µg/mL

-

Immunocytochemistry (ICC/IF)

1.0 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

1-20 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

ChIP assay (ChIP)

1-3 µL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HDAC2,uniProtId:Q92769-1,ncbiNodeId:9606,antigenRange:475-488,antigenLength:488,antigenImageFileName:PA1-861_HDAC2_Q92769-1_Rabbit.svg,antigenImageFileNamePDP:PA1-861_HDAC2_Q92769-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2118520

Product Specific Information

PA1-861 detects histone deacetylase 2 (HDAC2) from canine, hamster, human, mouse, non-human primate, rat tissues and cells.

PA1-861 has been successfully used in Western blot, ChIP, immunoprecipitation, immunofluorescence, flow cytometry, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing HDAC2 and a ~40 kDa protein of unknown identity from HeLa cell lysate. Immunocytochemical staining of HDAC2 in HeLa cells with PA1-861 results in nuclear staining.

The PA1-861 immunogen is a synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2. This immunizing peptide (Cat. # PEP-070) is available for use in neutralization and control experiments.

Target Information

HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.