Thermo Fisher Scientific NEFM Monoclonal Antibody (RMO-270)
Neurofilament medium protein(NF-M)을 인식하는 RMO-270 클론의 mouse monoclonal antibody. Western blot, IHC, ICC/IF 등 다양한 어플리케이션에 적합. 인간, 마우스, 랫트 등 여러 종에서 반응. 비인산화 epitope를 인식하며, 고순도 Protein A 정제된 액상형 항체.
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Thermo Fisher Scientific NEFM Monoclonal Antibody (RMO-270)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 29 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 15 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| ELISA | 0.1–0.5 µg/mL | – |
| Immunoprecipitation (IP) | 2–5 µg | – |
| Functional Assay | – | View 1 publication |
| In vitro Assay | – | View 1 publication |
| Miscellaneous PubMed | – | View 7 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Published Species | Avian, Chicken, Human, Mouse, Rat, Xenopus, Zebrafish |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RMO-270 |
| Immunogen | Rat neurofilaments |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2532998 |
Product Specific Information
- Cloning partner: Sp/2
- Reacts with the 160 kD polypeptide subunit of human neurofilament.
- Recognizes a phosphate-independent epitope in the tail (carboxy) domain of NF-M in most vertebrates and invertebrates.
- Suitable for immunohistochemical staining of Bouin’s and alcohol-fixed paraffin-embedded or frozen tissue sections.
- Recommended incubation: 30–60 minutes at room temperature or overnight at 4°C.
Target Information
Neurofilaments (NFs) are intermediate filaments expressed almost exclusively in neuronal cells, particularly in large axons.
They are composed of three polypeptide chains: NF-M (medium), NF-H (high), and NF-L (light).
These share a coiled-coil core domain but differ in N- and C-terminal sequences.
NF-M and NF-H have long, flexible tails that mediate filament interactions and cytoskeletal architecture.
NF-M phosphorylation and O-GlcNAcylation are reciprocally regulated, affecting translocation and filament formation.
Antibodies to NF subunits are valuable neuronal markers due to their abundance, neuron-specific expression, and biochemical stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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