Thermo Fisher Scientific NEFM Monoclonal Antibody (RMO-270)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 29 publications 29 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 15 publications 15 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.1-0.5 µg/mL

-

Immunoprecipitation (IP)

2-5 µg

-

Functional Assay (Functional)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 7 publications 7 publications

Product Specifications

Species Reactivity

Mouse, Rat, Human

Published species

Avian, Chicken, Human, Mouse, Rat, Xenopus, Zebrafish

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

RMO-270

Immunogen

Rat neurofilaments if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEFM,uniProtId:P12839-1,ncbiNodeId:10114,antigenRange:1-846,antigenLength:846,antigenImageFileName:13-0700_NEFM_P12839-1_House_mouse.svg,antigenImageFileNamePDP:13-0700_NEFM_P12839-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532998

Product Specific Information

The cloning partner for this antibody is Sp/2.

This antibody reacts with the 160 kD polypeptide subunit of human neurofilament. It specifically recognizes a phosphate-independent epitope in the tail (carboxy) domain of NF-M of most vertebrates and invertebrates.

This antibody is suitable for immunohistochemical staining of Bouin`s and alcohol-fixed paraffin-embedded or frozen tissue sections. To stain, incubate 30-60 minutes at room temperature or overnight at 4°C.

Target Information

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.