Thermo Fisher Scientific FOXC1/FOXC2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human FOXC1(Accession Q12948), corresponding to amino acid residues N118-L168. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FOXC1/FOXC2,uniProtId:Q12948-1,ncbiNodeId:9606,antigenRange:118-168,antigenLength:553,antigenImageFileName:PA5-101153_FOXC1FOXC2_Q12948-1_Rabbit.svg,antigenImageFileNamePDP:PA5-101153_FOXC1FOXC2_Q12948-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2850597

Product Specific Information

Antibody detects endogenous levels of total FOXC1/2.

Target Information

The forkhead transcription factor genes FOXC1 (Mf1) and FOXC2 (Mfh1) interact with the Notch signaling pathway and are required for the prepatterning of anterior and posterior domains in the presumptive somites through a putative Notch/Delta/Mesp regulatory loop. The genes have similar, dose-dependent functions, and compensate for each other in the early development of the heart, blood vessels and somites. Both FOXC1 and FOXC2 are expressed in the mesenchyme from which the ocular drainage structures derive. FOXC1 and FOXC2 also interact in kidney and heart development. Mutations in the FOXC1 gene result in Axenfeld-Rieger malformations of the anterior segment of the eye and lead to an increased susceptibility of glaucoma, including juvenile glaucoma. Functional regions in FOXC1 are required for nuclear localization and transcriptional regulation. Specifically, two regions in the FOXC1 forkhead domain, one rich in basic amino acid residues, and a second, highly conserved among all FOX proteins, are necessary for nuclear localization of the FOXC1 protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.