
Thermo Fisher Scientific CD309 (FLK1) Monoclonal Antibody (Avas12a1), Biotin, eBioscience
Mouse FLK1 (VEGFR2) 인식 단클론 항체로 혈관 내피 발달 연구에 적합. Biotin 결합형으로 Flow Cytometry에 최적화. Rat IgG2a, kappa 아이소타입이며 0.5 µg/test 사용 권장. 액상 형태로 4°C 암소 보관.
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Thermo Fisher Scientific CD309 (FLK1) Monoclonal Antibody (Avas12a1), Biotin, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 32 publications |
| Functional Assay | - | View 1 publication |
| Peptide Array | - | View 1 publication |
| Miscellaneous PubMed | - | View 7 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Dog, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Avas12a1 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466710 |
Available Formats
Product Specific Information
The Avas12a1 monoclonal antibody reacts with mouse Flk-1 (VEGFR2), a receptor tyrosine kinase involved in vascular endothelial tissue development. It is expressed on endothelial cells during embryonic stages and in some adult endothelial tissues.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on bEnd.3 cells; use ≤ 0.5 µg per test (100 µL final volume).
Cell number should be empirically determined (10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase that binds VEGF with high affinity. Expressed early in development by endothelial precursors, this receptor regulates vascular growth and signaling through multiple factors such as Rab GTPase, P2Y purine nucleotide receptor, integrin αVβ3, and T-cell protein tyrosine phosphatase. Mutations are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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