Thermo Fisher Scientific IL-10 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• In sandwich ELISAs, less than 1% cross-reactivity with recombinant canine IL-10 and recombinant porcine IL-10 is observed.
• Less than 0.4% cross-reactivity with recombinant human, mouse, rat, and feline IL-10 is observed.
• Reconstitute at 0.2 mg/mL in sterile PBS.
• Endotoxin level: <0.15 EU per 1 µg of antibody (LAL method).

Target Information

IL-10 encodes a cytokine primarily produced by monocytes, with some production by lymphocytes. It regulates immune responses and inflammation by:

• Down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages
• Enhancing B cell survival, proliferation, and antibody production
• Blocking NF-kappa B activity and regulating the JAK-STAT signaling pathway

Knockout studies in mice indicate IL-10 as an essential immunoregulator in the intestinal tract. Mutations in this gene are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.