Thermo Fisher Scientific Cystatin C Polyclonal Antibody
Thermo Fisher Scientific의 Cystatin C Polyclonal Antibody는 마우스 반응성 염기 항체로, WB, IHC, ELISA, IP 등에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS와 trehalose로 보존됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–15 µg/mL |
| ELISA | 0.1–0.4 µg/mL (Detection), 0.2–0.8 µg/mL (Capture) |
| Immunoprecipitation (IP) | 25 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Cystatin C (Ala21–Ala140) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609209 |
Product Specific Information
- In sandwich ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Cystatin D is observed.
- Less than 2% cross-reactivity with rhCystatin S/A, rhCystatin A, recombinant mouse (rm) Cystatin B, rhCystatin S/N, and rmCystatin E/M is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Cystatin C, a member of the type 2 cystatin superfamily, is a potent inhibitor of cysteine proteinases, including both endogenous lysosomal cathepsins and pathogenic proteases. It is a 13 kDa secreted protein found in high concentrations in biological fluids and expressed by all human nucleated cells.
Alterations in Cystatin C levels have been associated with cardiovascular diseases such as atherosclerosis, amyloid angiopathy, and myocardial infarction. Due to its low molecular weight, Cystatin C is also used as a marker of renal failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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