Thermo Fisher Scientific Calbindin D28K Monoclonal Antibody (4H7)
Calbindin D28K 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. Bovine, Human, Mouse, Pig, Rat에 반응. Protein A/G로 정제된 1 mg/mL 액상 형태이며, PBS와 50% glycerol buffer에 보존.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Pig, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4H7 |
| Immunogen | Full-length recombinant human calbindin protein expressed in and purified from E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 5 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2942420 |
Target Information
Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein or cholecalcin) is a highly conserved 28 kDa calcium-binding protein with broad tissue distribution.
It belongs to a family of low molecular weight calcium-binding proteins (CaBPs) including calmodulin, S-100, parvalbumin, and troponin C.
These CaBPs share homologous primary structures containing EF-hand motifs for Ca²⁺ binding.
Calbindin-D-28K is primarily found in subpopulations of neurons in the central and peripheral nervous systems, as well as epithelial cells involved in calcium transport such as distal tubular and cortical collecting tubule cells of the kidney, and enteric neuroendocrine cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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