Thermo Fisher Scientific GFP Monoclonal Antibody (3B11)
GFP 단백질을 인식하는 Mouse IgM 형 단일클론 항체로, WB, IHC, ICC/IF에 사용 가능. 비결합형 액상 형태로 제공되며, 높은 특이성과 재현성을 보장. 단백질 이동 및 발현 연구에 적합한 연구용 시약.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific GFP Monoclonal Antibody (3B11)
Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:5,000
Immunohistochemistry (IHC)
- Tested Dilution: 1:1,000–1:5,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:1,000–1:5,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3B11 |
| Immunogen | Recombinant protein purified from E. coli. The epitope is in the N-terminal 18 amino acids of the protein (MVSKGAELFTGIVPILIE), found in Clontech and other GFP vectors. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 5 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2942391 |
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP enables localization studies, movement tracking, and analysis of subcellular compartment dynamics. GFP technology has provided significant insights into the physiological activities of living cells.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without additional proteins, substrates, or cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications including:
- Monitoring gene expression and protein localization
- Studying protein-protein interactions (yeast two-hybrid system)
- Measuring distances between proteins (FRET experiments)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.