Thermo Fisher Scientific GFP Monoclonal Antibody (3B11)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:5,000

Immunohistochemistry (IHC)

• Tested Dilution: 1:1,000–1:5,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:1,000–1:5,000

Product Specifications

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.

As a fusion tag, GFP enables localization studies, movement tracking, and analysis of subcellular compartment dynamics. GFP technology has provided significant insights into the physiological activities of living cells.

GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without additional proteins, substrates, or cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications including:

• Monitoring gene expression and protein localization
• Studying protein-protein interactions (yeast two-hybrid system)
• Measuring distances between proteins (FRET experiments)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.