Thermo Fisher Scientific IgE Monoclonal Antibody (Ige21), NovaFluor Yellow 755, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
Monoclonal antibody IgE21 recognizes the immunoglobulin E (IgE) class of antibodies. IgE is present at low levels in serum and plays a key role in allergic responses by binding to mast cells and basophils via the Fc epsilon receptor I, leading to histamine and mediator release. IgE is produced by plasma B cells and basophils and exists in both soluble and membrane-bound forms. IgE21 recognizes both forms.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells. Use 5 µL (0.2 µg) per test in 100 µL final volume, with 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample before combining with cells.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures to minimize spillover and spread.

• Excitation: 552 nm
• Emission: 690 nm
• Laser: 561 nm (Yellow)

Target Information

IgE is one of the five immunoglobulin classes in mammals, composed of two epsilon heavy chains (each with 4 Ig-like constant domains) and two light chains. IgE mediates type I hypersensitivity reactions (e.g., hay fever, asthma, hives, anaphylaxis) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE-Fc complexes triggers degranulation and allergic responses. IgE also contributes to parasite immunity.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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