Thermo Fisher Scientific Ferritin Recombinant Rabbit Monoclonal Antibody (SC0620)
Ferritin 단백질 검출용 재조합 토끼 단클론 항체로, 높은 특이성과 재현성을 제공. Western blot, IHC, ICC/IF 등 다양한 응용 가능. HEK293 세포 발현 시스템 기반으로 생산된 고순도 항체. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC0620 |
| Immunogen | Synthetic peptide within Human FTH1 aa 58–99 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809530 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Ferritin is the major intracellular iron storage protein in both prokaryotes and eukaryotes. It consists of 24 subunits of heavy (H) and light (L) chains.
Key functions and characteristics:
- Stores iron in a soluble, non-toxic state
- Subunit composition affects iron uptake and release rates
- Defects in light chain gene linked to neurodegenerative diseases and hyperferritinemia-cataract syndrome
- Highly conserved protein essential for iron homeostasis
- Molecular weight ≈ 450 kDa, capable of storing up to 4,500 ferric iron atoms
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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