Thermo Fisher Scientific Phospho-N-WASP (Tyr256) Polyclonal Antibody

Applications

• Western Blot (WB): 1:1,000
• Immunocytochemistry (ICC/IF): 1:100

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to phosphotyrosine then affinity purified using phospho-N-WASP (Tyr-256) peptide (without carrier).
The antibody detects a 65 kDa protein corresponding to the molecular mass of phosphorylated N-WASP on SDS-PAGE immunoblots of A431 cells treated with pervanadate.
A similar band is observed in pervanadate-treated HeLa and endothelial cells.
Weak bands at higher molecular weights may be due to low cross-reactivity with phosphotyrosine.

Target Information

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structures and are involved in signal transduction from cell surface receptors to the actin cytoskeleton.
These proteins interact with multiple partners and are regulated by various stimuli.
They associate with the small GTPase Cdc42, which regulates actin filament formation, and the Arp2/3 complex.
The WASL gene product is a homolog of WAS protein, ubiquitously expressed with highest levels in neural tissues.
It directly binds Cdc42 and induces formation of long actin microspikes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.