Thermo Fisher Scientific Phospho-N-WASP (Tyr256) Polyclonal Antibody
Phospho-N-WASP (Tyr256)에 특이적인 Rabbit Polyclonal Antibody로 인간, 마우스, 랫트에 반응합니다. Western blot 및 ICC/IF에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 -20°C 보관하며, 연구용으로만 사용됩니다.
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Applications
- Western Blot (WB): 1:1,000
- Immunocytochemistry (ICC/IF): 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-N-WASP (Tyr-256) synthetic peptide (coupled to BSA) corresponding to amino acid residues around tyrosine 256 of human N-WASP. The human WASP sequence has a two amino acid difference in the same region surrounding tyrosine 291. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2943020 |
Product Specific Information
This antibody was cross-adsorbed to phosphotyrosine then affinity purified using phospho-N-WASP (Tyr-256) peptide (without carrier).
The antibody detects a 65 kDa protein corresponding to the molecular mass of phosphorylated N-WASP on SDS-PAGE immunoblots of A431 cells treated with pervanadate.
A similar band is observed in pervanadate-treated HeLa and endothelial cells.
Weak bands at higher molecular weights may be due to low cross-reactivity with phosphotyrosine.
Target Information
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structures and are involved in signal transduction from cell surface receptors to the actin cytoskeleton.
These proteins interact with multiple partners and are regulated by various stimuli.
They associate with the small GTPase Cdc42, which regulates actin filament formation, and the Arp2/3 complex.
The WASL gene product is a homolog of WAS protein, ubiquitously expressed with highest levels in neural tissues.
It directly binds Cdc42 and induces formation of long actin microspikes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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