Thermo Fisher Scientific ZO-1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Immunocytochemistry (ICC/IF)

1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Guinea pig / Ig

Class

Polyclonal

Type

Antibody

Immunogen

Human peptide antigen corresponding to ten residues at the splice junction. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZO-1,uniProtId:Q07157-1,ncbiNodeId:9606,antigenRange:1-1748,antigenLength:1748,antigenImageFileName:PA5-143805_ZO-1_Q07157-1_Guinea_pig.svg,antigenImageFileNamePDP:PA5-143805_ZO-1_Q07157-1_Guinea_pig_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_3092231

Product Specific Information

It cross reacts with mouse, rat and dog ZO-1 alpha-minus.

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.