Thermo Fisher Scientific ZO-1 Polyclonal Antibody
ZO-1 단백질을 인식하는 기니피그 폴리클로날 항체로, WB, IHC, ICC/IF 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트, 개 ZO-1에 교차 반응. 액상 형태로 제공되며, PBS + BSA 버퍼에 보존. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Guinea pig / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human peptide antigen corresponding to ten residues at the splice junction |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_3092231 |
Product Specific Information
This antibody cross-reacts with mouse, rat, and dog ZO-1 alpha-minus.
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. These proteins can also associate with claudin, occludin, and F-actin at tight junction strands, providing linkage between the actin cytoskeleton and the tight junction.
ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions; unlike ZO-1, ZO-2 is not expressed in nonepithelial tissue.
ZO-1 is located on the cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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