Thermo Fisher Scientific Rhesus/Cynomolgus Monkey TNF-alpha Recombinant Protein
Cynomolgus 및 Rhesus 원숭이 유래 TNF-alpha 재조합 단백질로, E. coli에서 발현된 고순도 (>90%) 단백질입니다. 세포독성 시험을 통해 생물학적 활성이 검증되었으며, 염증 및 면역 반응 연구에 적합합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific Rhesus/Cynomolgus Monkey TNF-alpha Recombinant Protein
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | Assay-dependent |
| Functional Assay | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Cynomolgus monkey, Rhesus monkey |
| Expression System | E. coli |
| Amino Acid Sequence | Cynomolgus/rhesus TNF, amino acids Val77-Leu233 (Accession # NP_001040614.1) with an N-terminal Met (Cynomolgus and rhesus TNF sequences are identical) |
| Molecular Weight | 17.4 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >90% by SDS-PAGE |
| Endotoxin Concentration | ng/µg |
| Activity | Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of actinomycin D. The ED50 is typically 2–20 pg/mL. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | 50 mM Tris (pH 8), 50 mM NaCl, 5% trehalose, 5% mannitol, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Product Specific Information
Reconstitution:
Centrifuge the vial briefly before opening. Reconstitute the lyophilized protein in sterile, distilled water to 0.1–1.0 mg/mL. Apportion into working aliquots and store at -20°C. Further dilutions should be made in low endotoxin medium or buffered solution containing carrier protein (e.g., heat-inactivated FCS or tissue culture grade BSA).Storage:
Store lyophilized protein at 2–8°C, preferably desiccated. Upon reconstitution, divide into aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
TNF-alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. Secreted mainly by macrophages, it binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors. TNF-alpha regulates immune cell activity, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It forms multimers (dimers, trimers, or pentamers) but appears as a single 17 kDa band under non-reducing SDS-PAGE conditions. Knockout studies suggest neuroprotective roles and tumor necrosis induction in mice. TNF-alpha also mediates immune responses to bacterial, viral, parasitic, and fungal infections, and contributes to tumor cell cytolysis—synergistic with interferon-gamma.
It has been implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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