Thermo Fisher Scientific Human CD3D His-tag/CD3E DYKDDDDK-tag Fc Chimera Recombinant Protein
Human CD3D/CD3E 재조합 단백질로, HEK293 세포에서 발현된 His-tag, Fc-tag, DYKDDDDK-tag 융합 단백질입니다. ELISA, Western blot, IHC 등 다양한 분석에 사용 가능하며, 고순도(≥90%) 및 낮은 엔도톡신(<1.0 EU/µg)을 보장합니다. 연구용으로만 사용됩니다.
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- Protein
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- pk
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | HEK293 cells |
| Amino Acid Sequence | Human CD3D (Met1–Ala105, Accession #P04234-1) with a C-terminal His-tagged human IgG1 Fc-tag coexpressed with human CD3E (Met1–Asp126, Accession #P07766-1) with a C-terminal His-tagged human IgG1 Fc-tag |
| Tag | DYKDDDDK-tag, Fc-tag, His-tag |
| Molecular Weight | 77.5 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥90% (SDS-PAGE), ≥95% (SEC-HPLC) |
| Endotoxin Concentration | <1.0 EU/µg |
| Activity | Binds OKT3-mIgG2a in a functional ELISA |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Product Specific Information
- Reconstitution:
Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower.
It is recommended to aliquot and use as soon as possible.
Store aliquots under sterile conditions at -20°C.
Avoid repeated freeze-thaw cycles.
Target Information
The CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and regulating the surface expression of the TCR complex.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of CD3 gamma, delta, epsilon, and zeta subunits, which are structurally related members of the immunoglobulin superfamily encoded on human chromosome 11.
These CD3 components have cytoplasmic tails that associate with signal transduction molecules, mediated by a double tyrosine-based motif.
CD3 may play a role in TCR-induced growth arrest, cell survival, and proliferation.
The CD3 antigen is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells.
Decreased T lymphocyte percentages may be observed in some autoimmune diseases.
Defects in the CD3 gene are associated with CD3 immunodeficiency.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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