Thermo Fisher Scientific Phospho-L-Plastin (Ser5) Polyclonal Antibody
Phospho-L-Plastin (Ser5) 폴리클로날 항체로, 인산화된 L-Plastin Ser5 잔기를 특이적으로 검출합니다. Western blot, IHC, ICC/IF에 적합하며, 인간, 마우스, 랫트 시료에서 반응합니다. 액상 형태로 -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human LCP1 (Accession P13796), corresponding to amino acid residues around phosphorylated Ser5. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817224 |
Product Specific Information
This antibody detects endogenous levels of Plastin L only when phosphorylated at Ser5.
Target Information
L-plastin is a leukocyte-specific actin bundling protein from the fimbrin family. It contains two actin-binding domains and two EF-hand type calcium-binding domains. Phosphorylation occurs on the serine residue in its headpiece region in response to inflammatory stimuli. L-plastin is hypothesized to regulate integrin-mediated adhesion in leukocytes and is expressed in most human cancer cell lines derived from solid tumors. Its expression is upregulated by testosterone in androgen receptor–positive prostate and breast cancer cells through androgen receptor binding elements upstream of the gene.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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