Thermo Fisher Scientific Phospho-CENTB1 (Ser554) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ACAP1(Accession Q15027), corresponding to amino acid residues around phosphorylated Ser554. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CENTB1,uniProtId:Q15027-1,ncbiNodeId:9606,antigenRange:554,antigenLength:740,antigenImageFileName:PA5-105821_CENTB1_Q15027-1_Rabbit.svg,antigenImageFileNamePDP:PA5-105821_CENTB1_Q15027-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2817220

Product Specific Information

Antibody detects endogenous levels of Centaurin beta-1 only when phosphorylated at Ser554.

Target Information

ACAP1, also known as Centaurin-beta-1 (CENTB1), is a 740 amino acid containing transport carrier belonging to the Centaurin family with three ANK repeats, an Arf-GAP domain, a BAR domain and a PH domain that binds to phospholipids. ACAP1, a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6), is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. It mediates CENTB1-binding to phosphatidylinositol 3,5-bisphosphate (PIP2) or phosphatidylinositol 3,4,5-trisphosphate (PIP3) containing membranes. Along with ACAP2, ACAP1 is recruited to platelet-derived growth factor (PDGF) induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, while over expression inhibited ruffle formation. It is widely expressed in most tissues with high expression in lung and spleen and low expression in heart, kidney, liver and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.