
Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17)
DYKDDDDK(FALG) 태그 단백질 검출용 recombinant rabbit monoclonal antibody. WB, ICC/IF, IP에 적합하며 높은 특이성과 재현성을 제공. Expi293 시스템에서 생산된 비결합 항체로, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 6 publications |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL | View 2 publications |
| Immunoprecipitation (IP) | 2.5 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8H8L17 |
| Immunogen | Synthetic peptide (DYKDDDDK) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532497 |
Additional Formats Available
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Biotin
- HRP
- Request custom conjugation
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
DYKDDDDK tag (FLAG tag) is an eight-amino-acid peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) used for protein purification, detection, and localization.
It can be fused to the N- or C-terminus of a protein to facilitate identification and isolation using anti-FLAG antibodies.
The tag’s hydrophilic nature minimizes protein denaturation or inactivation, making it widely used in molecular biology and biochemistry for:
- Protein-protein interaction studies
- Protein expression analysis
- Protein localization studies
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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