Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17)

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• Biotin
• HRP
• Request custom conjugation

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:

• Enhanced specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

DYKDDDDK tag (FLAG tag) is an eight-amino-acid peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) used for protein purification, detection, and localization.
It can be fused to the N- or C-terminus of a protein to facilitate identification and isolation using anti-FLAG antibodies.
The tag’s hydrophilic nature minimizes protein denaturation or inactivation, making it widely used in molecular biology and biochemistry for:

• Protein-protein interaction studies
• Protein expression analysis
• Protein localization studies

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.