Thermo Fisher Scientific B7-H4 Monoclonal Antibody (188), Functional Grade, eBioscience
Flow cytometry 및 기능적 분석에 사용 가능한 mouse B7-H4 단클론 항체. T세포 활성 억제 연구에 적합하며, 높은 순도(>90%)와 낮은 엔도톡신 수준(<0.001 ng/µg)을 보유. 4°C 보관, 액상 형태로 제공.
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4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific B7-H4 Monoclonal Antibody (188), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | View 1 publication |
| Neutralization (Neu) | Assay-Dependent | View 1 publication |
| Functional Assay (Functional) | Assay-Dependent | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 188 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2866149 |
Additional Formats
Product Specific Information
Description:
The 188 monoclonal antibody was generated against and reacts with mouse B7-H4 (also known as B7S1, B7X). Cross-reactivity with other proteins has not been determined. B7-H4 is a member of the B7 family that inhibits T-cell activation, cell cycle progression, and IL-2 production. The ligand for B7-H4 has not been identified. Double staining with antibodies Clone 9 and 188 indicates distinct epitopes or no steric hindrance. Clone 188 stains mouse B7-H4 transfected cells but not spleen cells. The expression pattern of B7-H4 remains incompletely characterized.
Applications Reported:
This antibody is reported for use in flow cytometric analysis and for blocking B7-H4 ligand binding in vitro.
Applications Tested:
Tested by flow cytometric analysis of transfected cells. Recommended ≤0.5 µg per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Titrate for optimal performance.
Storage and Handling:
Use in a sterile environment.
Filtration:
0.2 µm post-manufacturing filtered.
Purity:
90%, determined by SDS-PAGE.
Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).
Aggregation:
<10%, determined by HPLC.
Target Information
B7-H4 (B7x, VTCN1):
A costimulatory protein functioning as a negative regulator of T-cell mediated immunity. Although its receptor is unknown, B7-H4 delivers inhibitory signals preventing T-cell proliferation, cell cycle progression, and IL-2 production. It is expressed on activated T-cells, B-cells, monocytes, and dendritic cells. Aberrant expression is linked to lung, breast, and ovarian cancers. Overexpression may suppress T-cell immunity and promote tumor progression. VTCN1 may serve as a biomarker for early detection of ovarian cancer and renal cell carcinoma (RCC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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