Thermo Fisher Scientific H3K36me3 Polyclonal Antibody
H3K36me3에 특이적인 Rabbit Polyclonal Antibody로, Western blot, ICC/IF, ChIP assay에 사용 가능. 인간, 생쥐, 랫트 반응성. 액상 형태로 1 mg/mL 농도, -20°C 보관. Tri-Methyl-Histone H3 (Lys36) 검출에 적합한 연구용 시약.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ChIP assay (ChIP) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human HIST3H3 (Accession Q16695), corresponding to amino acid residues T23–Y42 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899177 |
Product Specific Information
Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys36.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo several types of post-translational modification, including methylation or acetylation of lysine and arginine residues and phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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